| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080768 |
| Chromosome: | chromosome 16 |
| Location: | 6061831 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676200 | (1 of 3) PTHR10751:SF2 - GUANYLATE-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAGCGAGGCAGGAGCAGGAGGAGAGTCG |
| Internal bar code: | CGCGCGCCATCGCGCCGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 99.1181 |
| LEAP-Seq n confirming: | 8092 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTACTCGCTGGGCTTG |
| Suggested primer 2: | TGTGACACCGGTACTCCGTA |