| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080779 |
| Chromosome: | chromosome 6 |
| Location: | 5578359 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285500 | (1 of 2) K13173 - arginine and glutamate-rich protein 1 (ARGLU1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGCGGCGGGTTCGGCCGCGTCGCCCA |
| Internal bar code: | ATGGCGGATCTGAGGGGCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 99.1961 |
| LEAP-Seq n confirming: | 8021 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGAAGCCATACTTCCTC |
| Suggested primer 2: | GGGATTGGCTGTGATTGAGT |