| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080781 |
| Chromosome: | chromosome 9 |
| Location: | 7927171 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g417250 | ELG27 | Exostosin-like glycosyltransferase 27; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCAAGGGCGAGCGCACACAAGGGAGGC |
| Internal bar code: | TAGTCAAGGGGTCAGGTTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.0244 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAAGCATGCTGGAAGTT |
| Suggested primer 2: | ACTGCGGGACAAGAGAAAGA |