| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080781 |
| Chromosome: | chromosome 14 |
| Location: | 2724028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626800 | OPR64 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGATTACGCCAGTCCCCGGGGATCTGCC |
| Internal bar code: | GCGTCGACGGCTATAAGGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 91.6874 |
| LEAP-Seq n confirming: | 1103 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGAACGCAAGCGTGTAAC |
| Suggested primer 2: | TCACTGTGCTCGCCATACA |