| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.080783 |
| Chromosome: | chromosome 12 |
| Location: | 1775442 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512300 | (1 of 1) 1.13.11.58 - Linoleate 9S-lipoxygenase / Linoleate 9-lipoxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGGGGGGCGGCAGCTCGCGGTCGGGGG |
| Internal bar code: | CGTGTCTCGGCTGACCGCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 98.6842 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATAATGGCGCAGTGGGTT |
| Suggested primer 2: | CCACCCACCTAACCATTACG |