| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.080799 |
| Chromosome: | chromosome 16 |
| Location: | 974790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648900 | (1 of 2) PF02696 - Uncharacterized ACR, YdiU/UPF0061 family (UPF0061) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATGCATGTACAACGTGAGTATACTGTA |
| Internal bar code: | ATTAGGCACTTAACCAAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.6689 |
| LEAP-Seq n confirming: | 8729 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCGTACACTGACGCGAT |
| Suggested primer 2: | CTGACGTACGACATTGGTGG |