Insertion junction: LMJ.RY0402.080816_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.080816
Chromosome:chromosome 4
Location:1499026
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g213905 GEX148 (1 of 2) K13091 - RNA-binding protein 39 (RBM39, RNPC2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCCATGGTTGCAAAGTCGTGCTTGAAT
Internal bar code:GCGCTTTCCCGCCCGACAATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:19.863
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:234
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCTTTAACACACGTCGG
Suggested primer 2:GGAGAAGAGGTGCAACGAAG