| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080816 |
| Chromosome: | chromosome 4 |
| Location: | 1499618 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213905 | GEX148 | (1 of 2) K13091 - RNA-binding protein 39 (RBM39, RNPC2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCACCTCTTCTCCTTGCCACGCTTCAT |
| Internal bar code: | GATATTCGAGCCGCTCGTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 99.0018 |
| LEAP-Seq n confirming: | 9521 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGTATGTGTGGTGTGGT |
| Suggested primer 2: | GTTGCATGCCCCTTACAGTT |