| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080842 |
| Chromosome: | chromosome 7 |
| Location: | 1466970 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323500 | (1 of 1) PF08609 - Nucleotide exchange factor Fes1 (Fes1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCTGCGGCGTTCCCGCGCCCCCACGGC |
| Internal bar code: | CGGTTATGACCGGAACGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 94.0299 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | CTCCACTCCCACACACACAC |