Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.080842 |
Chromosome: | chromosome 7 |
Location: | 1467045 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g323500 | (1 of 1) PF08609 - Nucleotide exchange factor Fes1 (Fes1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCATGCTGGCCCCGCAGGTGTCGGCGG |
Internal bar code: | GGCGCCTAATTAGGGGCTTTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 350 |
LEAP-Seq percent confirming: | 99.6946 |
LEAP-Seq n confirming: | 13712 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
Suggested primer 2: | CTCCACTCCCACACACACAC |