| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080864 |
| Chromosome: | chromosome 4 |
| Location: | 1359960 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214501 | PNP1,PNT2 | Chloroplast polyribonucleotide phosphorylase; (1 of 1) 2.7.7.8 - Polyribonucleotide nucleotidyltransferase / Polynucleotide phosphorylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGTGGCGACACTGGGCAGTGCCAACG |
| Internal bar code: | AGCCCAGGGTTAAAAACGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 99.6278 |
| LEAP-Seq n confirming: | 1606 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGCTGAGTAAGGAGGTG |
| Suggested primer 2: | TGCGTTTCCCTAACCGTAAC |