| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080896 |
| Chromosome: | chromosome 6 |
| Location: | 4022734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278205 | (1 of 1) PTHR22838:SF4 - WD REPEAT-CONTAINING PROTEIN 13 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGGATACTGGTCCCTTTTGACCAGAGG |
| Internal bar code: | AAATACTTGCAGTTGTAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 98.9264 |
| LEAP-Seq n confirming: | 645 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCGGCAAAGAGCTTCAG |
| Suggested primer 2: | CCCATACACGTTCACTCACG |