Insertion junction: LMJ.RY0402.080941_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.080941
Chromosome:chromosome 14
Location:449848
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g610900 (1 of 1) IPR026205 - Progesterone-induced-blocking factor 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAGGGACCTTGCCGGGCATGGCCCAGAC
Internal bar code:GGAGGTTCCAGACGGAGCCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:99.2879
LEAP-Seq n confirming:976
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGCATGCACAAATGAAT
Suggested primer 2:ATCAGTAACACCTCCACGGC