Insertion junction: LMJ.RY0402.080941_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.080941
Chromosome:chromosome 14
Location:449944
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g610900 (1 of 1) IPR026205 - Progesterone-induced-blocking factor 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGTCCCTCGGTCCCTCCCTCGGTCCTC
Internal bar code:ATGTGCGCTATGTGTTTGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:79.2453
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGCATGCACAAATGAAT
Suggested primer 2:GACCTGCACCACACAATCAG