| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.080968 |
| Chromosome: | chromosome 8 |
| Location: | 559681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359400 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTGCAGCAGCTCATGTCCTCACCTGAG |
| Internal bar code: | GCAGGGCATAGTTCTCAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 75.6048 |
| LEAP-Seq n confirming: | 375 |
| LEAP-Seq n nonconfirming: | 121 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACATTCCTTGAAGCCC |
| Suggested primer 2: | TGGGAAATCAAGGCTTTACG |