| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080969 |
| Chromosome: | chromosome 1 |
| Location: | 4731400 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032650 | TAL1 | Transaldolase; (1 of 1) PTHR10683//PTHR10683:SF6 - TRANSALDOLASE // TRANSALDOLASE-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTAGCGCTACGGAGCCATGCGCGTGAC |
| Internal bar code: | GATGATGCTCGCGGAGCATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 716 |
| LEAP-Seq percent confirming: | 99.5098 |
| LEAP-Seq n confirming: | 2436 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTTTGCTCAGGCCCTAC |
| Suggested primer 2: | TGTGGCCCCTTCCTACTATG |