| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.080974 |
| Chromosome: | chromosome 5 |
| Location: | 2687385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236650 | CYG63 | (1 of 5) IPR001054//IPR006189//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // CHASE domain // Nucleotide cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTGGTAGTGGCGGTGGTGATCTGCCT |
| Internal bar code: | ATCTGGTCCCGGAAGCTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 75.0056 |
| LEAP-Seq n confirming: | 3343 |
| LEAP-Seq n nonconfirming: | 1114 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACACCTGCACGTTGGTC |
| Suggested primer 2: | ACCTCACCACTTCACGAACC |