| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081004 |
| Chromosome: | scaffold 18 |
| Location: | 139877 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749147 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGGGCGAGGGGGAGGTGTTGTGATGCG |
| Internal bar code: | CCCTGGAGCAGAGAACAGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 87.2558 |
| LEAP-Seq n confirming: | 2814 |
| LEAP-Seq n nonconfirming: | 411 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGACTGGGAGTTGTCGCT |
| Suggested primer 2: | ACGCACACCTGTTTGAAGTG |