| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081009 |
| Chromosome: | chromosome 14 |
| Location: | 561197 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611700 | PLAP8,PLP8 | Plastid lipid associated protein 8; (1 of 1) PF01476//PF04755 - LysM domain (LysM) // PAP_fibrillin (PAP_fibrillin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTGCATTGCACCCCTGACAGCGCAGT |
| Internal bar code: | CTCGTTACAATGGCCCGAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1114 |
| LEAP-Seq percent confirming: | 98.8798 |
| LEAP-Seq n confirming: | 6620 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTATGTTAGGTGGCAGT |
| Suggested primer 2: | TTCTGTTGTGCGATCTCCAG |