| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081010 |
| Chromosome: | chromosome 8 |
| Location: | 4875300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384864 | (1 of 1) PF00018//PF14604 - SH3 domain (SH3_1) // Variant SH3 domain (SH3_9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCACCACCTCTCCCCCTCCTCCACCC |
| Internal bar code: | CTTCTCGGTAACGTCTTGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 99.3888 |
| LEAP-Seq n confirming: | 12034 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACTACGGCGACTATGGC |
| Suggested primer 2: | TGATTCAGGACACAGGACCA |