| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081022 |
| Chromosome: | chromosome_5 |
| Location: | 1957734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g241637 | FAP32 | Intraflagellar transport protein 46 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTACAGTGTGAACAGCACGTGCAGGCTCT |
| Internal bar code: | TGACTCCAACGGAGCCCTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 98.1021 |
| LEAP-Seq n confirming: | 2326 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAACGACATCCACAAGG |
| Suggested primer 2: | CCTGAAATTTGAACCCCTCA |