| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081025 |
| Chromosome: | chromosome 6 |
| Location: | 5175507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281600 | LCI23 | Low-CO2-inducible protein 23 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGCCGACAAAGCGCATCCGCATGTCCA |
| Internal bar code: | ATTTCTAGCCGATGTTGTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.5006 |
| LEAP-Seq n confirming: | 4383 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGGTTAACAGGGGTCA |
| Suggested primer 2: | GGATGTGTGCGTGGTTGTAG |