| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081064 |
| Chromosome: | chromosome 8 |
| Location: | 1170155 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362950 | (1 of 3) IPR002202 - Hydroxymethylglutaryl-CoA reductase, class I/II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTCCCTGCTGCCCTATTCCCGTGCCAC |
| Internal bar code: | CTCTCATTGCGTCTAGCAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 56.8182 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCGCTGAACAAGACATC |
| Suggested primer 2: | GGTGGGAGAAGGAGAAGGAG |