| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081072 |
| Chromosome: | chromosome 6 |
| Location: | 5295475 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282900 | (1 of 1) K09288 - coiled-coil domain-containing protein 6 (CCDC6, PTC) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGGCAGCCTCCGCAATTCCAAGCTTC |
| Internal bar code: | ACTGAGGATCGTCTCTGACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 90.3704 |
| LEAP-Seq n confirming: | 610 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGCAAGGTTGATTGGA |
| Suggested primer 2: | ACACCAAGCCATATCCAAGC |