Insertion junction: LMJ.RY0402.081082_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.081082
Chromosome:chromosome_14
Location:2671028
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre14.g626466 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TAAACTTAATGCATGCGCGCCCCCCCCCCC
Internal bar code:CACCAACTCCGAGGGGTGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:330
LEAP-Seq percent confirming:97.6023
LEAP-Seq n confirming:692
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGAACCAAACTAACCCTGGC
Suggested primer 2:CTGTCTAGAATTGCGCCTCC