| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081082 |
| Chromosome: | chromosome 14 |
| Location: | 2671028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626466 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACTTAATGCATGCGCGCCCCCCCCCCC |
| Internal bar code: | CACCAACTCCGAGGGGTGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 97.6023 |
| LEAP-Seq n confirming: | 692 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACCAAACTAACCCTGGC |
| Suggested primer 2: | CTGTCTAGAATTGCGCCTCC |