Insertion junction: LMJ.RY0402.081087_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.081087
Chromosome:chromosome 9
Location:142350
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g386732 (1 of 1) 2.4.1.57 - Phosphatidylinositol alpha-mannosyltransferase / Phosphatidyl-myo-inositol alpha-mannosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTGCCACTGGCCCGCCTAACAGTCAACT
Internal bar code:ATCGTCGCATCCGTCTCGAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:169
LEAP-Seq percent confirming:99.8134
LEAP-Seq n confirming:1605
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGACATCTACATCCACCA
Suggested primer 2:ACCGCCTTGTTGTCGTACTT