| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081088 |
| Chromosome: | chromosome 6 |
| Location: | 2804781 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271200 | PNO1 | (1 of 1) 1.6.3.3 - NADH oxidase (H(2)O(2)-forming) / H(2)O(2)-forming NADH oxidase; Pyridine nucleotide-disulphide oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATGCATGTGCGAGCCGCAAGAGGTGAT |
| Internal bar code: | TTAAGTTCAATCCTGGCGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.6441 |
| LEAP-Seq n confirming: | 280 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGAGCAGGGAGATGGAG |
| Suggested primer 2: | GCCATCTCAGATTCTCAGCC |