Insertion junction: LMJ.RY0402.081102_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.081102
Chromosome:chromosome 9
Location:4154099
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g393954 FAP106 Flagellar Associated Protein 106; (1 of 1) PTHR21490:SF0 - ENKURIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGGGGCGGTCACTTTGGGCTCAGTGAA
Internal bar code:CGGCGCCTAGAAGCAGCTGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:51.2304
LEAP-Seq n confirming:229
LEAP-Seq n nonconfirming:218
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGAGTGTGGACAGTGCT
Suggested primer 2:CTATTGGTGTGCGTGTGGTC