Insertion junction: LMJ.RY0402.081117_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.081117
Chromosome:chromosome 4
Location:2846191
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g224250 FAP147 Flagellar Associated Protein 147; (1 of 1) PF14646 - MYCBP-associated protein family (MYCBPAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCACGTTTACTTGTCAGCATCATGGTG
Internal bar code:GGTGAGCGACATTTACGCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:501
LEAP-Seq percent confirming:99.5656
LEAP-Seq n confirming:6647
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GAAACAAGTCGCTTTAGCCG
Suggested primer 2:CAGCCACCAATGCACATAAG