| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081117 |
| Chromosome: | chromosome_4 |
| Location: | 2846191 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224250 | FAP147 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGCACGTTTACTTGTCAGCATCATGGTG |
| Internal bar code: | GGTGAGCGACATTTACGCTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.5656 |
| LEAP-Seq n confirming: | 6647 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACAAGTCGCTTTAGCCG |
| Suggested primer 2: | CAGCCACCAATGCACATAAG |