| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081143 |
| Chromosome: | chromosome_6 |
| Location: | 5603124 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g285700 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCCAAGCAGGTGCGCACTTTGGCAGTTTG |
| Internal bar code: | TTTTCACTCACACGAGTACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 73.8309 |
| LEAP-Seq n confirming: | 1642 |
| LEAP-Seq n nonconfirming: | 582 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACGAAAAAGGACCAGTG |
| Suggested primer 2: | ACTTGGTCTGGATTTGACGC |