| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081152 |
| Chromosome: | chromosome_3 |
| Location: | 5547828 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g186300 | p38,DII2,FAP146 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGCTCGTTTCTGGCGCCTGGAGTACGTG |
| Internal bar code: | CGAAAGGGATTTGCGAAGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 94.7418 |
| LEAP-Seq n confirming: | 2991 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGGCTGTGTTGCTCTCT |
| Suggested primer 2: | GCAGTTTGGCAGTGTTGAGA |