Insertion junction: LMJ.RY0402.081164_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.081164
Chromosome:chromosome 4
Location:3871810
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g230340 FAP206 (1 of 1) PTHR21442:SF0 - UPF0704 PROTEIN C6ORF165; Flagellar Associated Protein 206 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCATCTTGGCGTCTGCCGCACCTCGCAGC
Internal bar code:CTGTTTGATTCCGTAGCCGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:217
LEAP-Seq percent confirming:98.3927
LEAP-Seq n confirming:1408
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACAGAGCTTGGC
Suggested primer 2:AGCTAATGGGGTTTGTGTGC