| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081165 |
| Chromosome: | chromosome 2 |
| Location: | 1120578 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081350 | (1 of 1) PTHR11080//PTHR11080:SF2 - PYRAZINAMIDASE/NICOTINAMIDASE // PROTEIN PNC-1, ISOFORM A; Nicotinamidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCCGAGACTCGAGAGGAACGTGTCGCG |
| Internal bar code: | GACCGCGTTCATTCGGCTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 79.2774 |
| LEAP-Seq n confirming: | 1163 |
| LEAP-Seq n nonconfirming: | 304 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCGACACGATTTACCTC |
| Suggested primer 2: | GTTGTTGCAGGATGGGAAGT |