| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.081168 |
| Chromosome: | chromosome_17 |
| Location: | 4112631 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g729550 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCACACACCGGGTGCACGCCAGGTCACA |
| Internal bar code: | ACGCGGGGAAATACACTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.5792 |
| LEAP-Seq n confirming: | 3786 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAACCCCTCAACACACT |
| Suggested primer 2: | CAAAGTTGTCTCTCACGCCA |