| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081169 |
| Chromosome: | chromosome_5 |
| Location: | 1319660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g244800 | NUOAF3 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCAGCTGGATGCCCTCGTAGGGGCTCAGG |
| Internal bar code: | TGTCCGTCAGCCACTTGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 96.0448 |
| LEAP-Seq n confirming: | 1457 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAGGGATGAGAGGGGAG |
| Suggested primer 2: | ATCCCAATACACTCCCCCTC |