| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081200 |
| Chromosome: | chromosome 1 |
| Location: | 5551500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039400 | (1 of 1) 3.8.1.5 - Haloalkane dehalogenase / 1-chlorohexane halidohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCCGTTGTCCGGAGATACCAAGCACGT |
| Internal bar code: | GCGGGGCGCCCTGCGGCCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 4.45545 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAGAATTAAGCAACGGC |
| Suggested primer 2: | TTGAGGTTCCCAGGTATTCG |