| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081216 |
| Chromosome: | chromosome 3 |
| Location: | 1174996 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149450 | (1 of 1) IPR006037//IPR016040 - Regulator of K+ conductance, C-terminal // NAD(P)-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGTGCGCTCCTGCACTGCCGTCGCAAAT |
| Internal bar code: | GTGCGTGTAGGACGCCGTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 320 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAGGGGGTGTCTGGTGAG |
| Suggested primer 2: | ACAGGTTGGGTGACAGGAAG |