| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081238 |
| Chromosome: | chromosome 16 |
| Location: | 4171276 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668850 | A2,MTA2 | Hydroxyproline-rich glycoprotein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACGACTGCAGAAGTCGTACCGCGGCTTG |
| Internal bar code: | CTAGGGATAATGGTCCCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 593 |
| LEAP-Seq percent confirming: | 98.8646 |
| LEAP-Seq n confirming: | 2264 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCATTTTACCGCTCTGG |
| Suggested primer 2: | CAGCAGGCGGTATCTCTAGG |