Insertion junction: LMJ.RY0402.081258_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.081258
Chromosome:chromosome 6
Location:6964860
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g296350 SENP5,ULP1 ULP2-type SUMO protease; (1 of 5) 3.4.22.68 - Ulp1 peptidase / Ulp1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGCCGCCCGCCGCCACCAGCGCGGCCAC
Internal bar code:GTTGGAGGCAAGTAAAGGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:64
LEAP-Seq percent confirming:98.4615
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCCATACCTCGAAGCCT
Suggested primer 2:CCAGCCCAAACTAAACCAAA