| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081290 |
| Chromosome: | chromosome 8 |
| Location: | 1671318 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365720 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGTGGGGCTGGCGGCGGTGGAGGCGG |
| Internal bar code: | ACTATTATAACTGAGATAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1187 |
| LEAP-Seq percent confirming: | 99.0426 |
| LEAP-Seq n confirming: | 8379 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCAGCTATACCAGTAGGC |
| Suggested primer 2: | TTCTTAACACACGCAAACGC |