| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081311 |
| Chromosome: | chromosome_9 |
| Location: | 6976744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g410700 | MDY5,MDH5 | NADP-dependent malate dehydrogenase, chloroplastic | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGCGATCCCTGCCTCCAGGTGTCCATCGG |
| Internal bar code: | CTTGCTCGGCGACCAACCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 98.9628 |
| LEAP-Seq n confirming: | 1145 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGACCGAGGTGTAGAAC |
| Suggested primer 2: | AAGTACCCAGCAAACATGGC |