Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.081313 |
Chromosome: | chromosome_16 |
Location: | 1173966 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g650600 | MST1 | Mastigoneme-like flagellar protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCCGGCACCGTGTGCCTGCCCTGCCCCTC |
Internal bar code: | GGACCGATGGATAGTGGAGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 185 |
LEAP-Seq percent confirming: | 90.5447 |
LEAP-Seq n confirming: | 881 |
LEAP-Seq n nonconfirming: | 92 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTTACACCGGCCAGAATGT |
Suggested primer 2: | GCTGCCTAATTCCGTGAGAG |