| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081374 |
| Chromosome: | chromosome 13 |
| Location: | 158257 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563200 | HAD7 | Haloacid dehalogenase-like hydrolase; (1 of 1) PTHR12725:SF63 - ALPHA-D-GLUCOSE-1-PHOSPHATE PHOSPHATASE YIHX | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGCACGATGGCAAAAGCGCTGACACGA |
| Internal bar code: | CCTCGGGATGGGGGGTCAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.9057 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCCTGGAGCTTTGTAAG |
| Suggested primer 2: | TGTAATTGAGAGCACGCAGG |