| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081394 |
| Chromosome: | chromosome 17 |
| Location: | 2597795 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAACTAAAAGCTGAATTCGAAGCGAAGC |
| Internal bar code: | CTGACAGGCTGGCAGGCTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.5883 |
| LEAP-Seq n confirming: | 4112 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTTGAGGTCGTGTTGA |
| Suggested primer 2: | AAGAAGTGAAGGCGTAGCCA |