Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.081410 |
Chromosome: | chromosome 10 |
Location: | 1618704 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g429601 | (1 of 2) PTHR10060:SF22 - CELL DEATH-RELATED NUCLEASE 2 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCAGAGCATGCAGCAGTCTTGGAAATGC |
Internal bar code: | ATATTGGCTTTCAAACGAGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 738 |
LEAP-Seq percent confirming: | 99.7643 |
LEAP-Seq n confirming: | 12275 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAACCAACCCCAATGCAATA |
Suggested primer 2: | TGACGAGCACTATCACTCCG |