| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.081446 |
| Chromosome: | chromosome 1 |
| Location: | 927913 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005001 | CPLD9 | Conserved in the Plant Lineage and Diatoms | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGCGCGCAACAGCATTTCAGCCCGCA |
| Internal bar code: | CGAACGTTTTCTGACCGTCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1043 |
| LEAP-Seq percent confirming: | 99.5246 |
| LEAP-Seq n confirming: | 5234 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTCAGGAAGTTCTTGCG |
| Suggested primer 2: | AGACCCTGTGTTCCGTCAAC |