| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.081456 |
| Chromosome: | chromosome 10 |
| Location: | 2169466 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433650 | TMC3 | (1 of 4) 2.1.1.178 - 16S rRNA (cytosine(1407)-C(5))-methyltransferase; putative tRNA (cytosine-5)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTACGCAGGCGTGAGTCCATGTCCTAC |
| Internal bar code: | CCGCGGGAACACATCCGCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 98.7138 |
| LEAP-Seq n confirming: | 614 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGGAGCAAGGTCTGGAG |
| Suggested primer 2: | TTATGGAAACCAAACCCCAG |