| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081459 |
| Chromosome: | chromosome 3 |
| Location: | 3461577 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167450 | (1 of 22) PF13640 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGATCAGGGTGCCTGTGAATGCCACAC |
| Internal bar code: | GGAACGTGCGAAGGACAGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 67.365 |
| LEAP-Seq n confirming: | 1447 |
| LEAP-Seq n nonconfirming: | 701 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCCTTCTTGCTACGC |
| Suggested primer 2: | CACGTGTGTGTTTCTCCACC |