Insertion junction: LMJ.RY0402.081467_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.081467
Chromosome:chromosome 2
Location:4175323
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g098350 (1 of 1) PTHR11709//PTHR11709:SF118 - MULTI-COPPER OXIDASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGTGAGTGCGGTTGGTGTGACTTGGCTT
Internal bar code:CCTTCGAAGGCACATGGGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:530
LEAP-Seq percent confirming:98.9045
LEAP-Seq n confirming:632
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTGTATGCTCTTTGCGT
Suggested primer 2:TGGAGCAGCAGTCATAGGTG