| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.081477 |
| Chromosome: | chromosome 2 |
| Location: | 6506388 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116550 | DNJ24 | (1 of 1) PF00226//PF08240 - DnaJ domain (DnaJ) // Alcohol dehydrogenase GroES-like domain (ADH_N); DnaJ-like protein | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGGCGCGCCTACCGCAACCTGCTGACA |
| Internal bar code: | AAGCCGGCATGGGCCTTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 98.8465 |
| LEAP-Seq n confirming: | 3342 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAAAGCGTAGCCGTATC |
| Suggested primer 2: | ACACCATGTGGCATGTGAAC |